SEROLOGICAL TEST FOR HBsAG AND HBcAG
AIM:
To detect the presence of HBsAg and HB
c Ag
in the given clinical samples.
INTRODUCTION:
HBcAg is abbreviated to Hepatitis B core antigen. Similarly
HBsAg is abbreviated to Hepatitis B surface antigen. As the name indicates both are antigens and they
belong to Hepatitis B virus. Hepatitis B virus is a double stranded DNA virus. It is responsible for causing liver infection. Presence of HBcAg in blood reveals the presence of virus and active replication of
virus. HBsAg can be detected with blood
test. The mode of transmission is through
virus contaminated blood and body fluids.
MATERIALS REQUIRED:
Antigen kits, Serum
or Plasma,
Cassette, Dispenser,
Grease free glass slides
PROCEDURE:
1. Before testing,
make sure the sample and
test kits are at room temperature.
2. Remove the
cassette from
the
foil-wrapped pouch by opening it.
3. The cassette should be put on a smooth, clean surface. Test right away after
opening.
4. Transfer 3 drops of the serum or plasma (about 75 ยตl) using the provided dropper to the sample well on
the cassette.
5. Do not contain air
bubbles.
6. Wait for 15
minutes and then, read the results.
7. Results obtained more than 20 minutes are seemed invalid and After finishing the test
dispose of the cassette carefully.
RESULT:
For Positive result, there is formation of band at both the regions. For negative result, there is formation of band at Control region only our result in which we band formation only at control region. The person is not affected by Hepatits virus. It can cause chronic Infection. HBsAg test can detect the presence of hepatitis virus in blood. If the test gets positive, the person is infected and then further test is A this is a need to determine acute or chrome hepatitis B infection. HBCAg can be found in needles of liver cells, no free HBCAg in serum. HBCAg the marker of replication of HBV HBC-IgM that is typically positive with acute HBV infection is not generally required to diagnose active infection.
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