REAL TIME PCR AIM: Demonstration of Real-Time PCR through experimental samples INTRODUCTION: Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation ofnucleic acids. It monitors the amplification of a targeted DNA molecule during the PCR (i.e.in real time), not at its end, as in conventional PCR. Real-time PCR is the technique ofcollecting data throughout the PCR process as it occurs, thus combining amplification and detection into a single step. Using PCR, specific sequences within a DNA or cDNA template can be copied, or “amplified”, many thousand- to a million-fold using sequence specific oligonucleotides, heat stable DNA polymerase, and thermal cycling. The PCR is the cyclic reaction based on the rapid change in temperature during each step. During PCR, our gene of interest is amplified as well as quantified. Real-time quantitative PCR is the reliable detection and measurement of products generated during each cycle of...