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Showing posts from August, 2023

MICROBIAL DYE DECOLOURIZATION - METHYLENE BLUE

  MICROBIAL DYE DECOLOURIZATION - METHYLENE BLUE   AIM: To determine decolourise the ability of given Microorganisms to degrade the synthetic dyes               INTRODUCTION: Synthetic dyes have largely replaced natural dyes over the years. It is estimated that around 10,000 different dyes are currently being produced on an industrial scale, with an annual worldwide. Production volume of around 10,000 tons and about 10 to 15% of those are discarded into nature. When effluents come from dye-based industries like textile industries seems as an principle source and is one of the most threatening our biodiversity. Generally, textile dyes are Mostly aliened into azo, reactine   Hiphenylmethane, heterocyclic, polymeric composition Among etc. all the textile dyes, azo dyes are most suitable to use in the textile industry they are very stable. To light, water, heat, bleach, detergents, and perspiration due to their quality and A con...

ISOLATION Of AIR BORNE MICROORGANISM-SETTLING PLATE METHODS

  ISOLATION Of AIR BORNE MICROORGANISM-SETTLING PLATE METHODS   AIM : To isolate the air borne microorganisms by specific techniques. PRINCIPLE : The Isolation of Microorganism From Air is performed by using the settle-plate technique. In this method a suitable medium is poured over a sterile petri dish and then allow it to slidify. After that the plate is exposed to the open air for a few minutes. Then the plates are incubated for the formation of microbial colonies on the plate. Observe the colonies by using a microscope. Czapek medium, also known as Czapek’s agar (CZA) or Czapek-Dox medium. This media is used for propagating fungi and other organisms.Czapek medium is composed of   iron sulfate(0.01g), potassium chloride (0.5g),   magnesium sulfate(0.5g), monopotassium phosphate(1g), cane sugar(30g),   sodium nitrate (2g) and distilled water (1000 lit).The   Czapek-Dox agar is an optimum growth medium for Aspergillusbrasiliensis, Candida albic...