ANIMAL HANDLING DATA and REQUIRMENT FOR VIROLOGY LAB

Life span of animals: 

Daily food intake:

Physiological data:

Basic requirements for lab animals:

Requirement of the virology lab:

 (1) Physical infrastructure.

 (2) Human resources.

 (3) Equipment and supplies.

Physical infrastructure:

Essential equipment:

•   Biosafety cabinets Class II – Three (one for handling cell cultures, one for handling stock viruses, and one for processing clinical specimens).
•   One incubator and two CO 2 incubators (one for uninfected cell cultures and the other for infected cell cultures). 
•  20 ° C and –70 ° C freezers. 
•  Inverted light microscope. 
•  Fluorescent microscope with photography attachments. 
•  Filtration apparatus for preparation of tissue/cell culture media. 
•  Refrigerate centrifuge.
•   water bath.
•   pH meter.
•  Magnetic stirrer.
•  Vortex mixer.
•   Elisa Reader and washer.
•   Micropipettes ( 100 u l, 200 u l, 20 u l).
•  Hot air oven for sterilizing glassware.
•  PCR machine.
•  Gel electrophoresis apparatus.
•  UV transilluminator.
•  liquid nitrogen container.