DR . R. MAHENDIRAN RAMASAMY

    I MMUN O D I FFU S I ON TE C H N I Q U E - O UC HTE R LO N Y M ETHOD   AI M :    Und e rst a nd the a nt i g e n a nt i bo d y in t e ra c t i ons a nd f a m i l i a r w i t h dif fe r e nt a n t i g e ns n a tu r e in a mi x tur e .   P RI N C I P LE:   This method w a s fi r st in t rodu c e d b y O r jan Ou c h t e rlo n y . Th e r e a re two t y p e s of a nt i g e ns such a s soluble a nt i g e n a nd sur f a c e a nt i g e n. B a s e d on the p h y si c a l a nd c h e m i ca l n a ture of the a nt i g e n bio l o g ic a l re ac t i on a g g lu t ination a nd p r e c ip i tation would o cc u r . The soluble a nt i g e n a nd r e spe c t i ve a nt i bo d y in a g a rose g e l w e l l , t h e y d i f f use r a dial l y a nd r e sult a p r ec ip i tation b a nd b e tw ee n the w e l l s. M AT E RI A LS REQUI R ED:   Anti g e ns, a nt i s e rum, a g a ros e , w e ll ...

  D OT E L I SA   A I M :   To le a rn the te c hnique Dot E LI S A for the d e te c t i on of a nt i g e n or a nt i b od y in the g iven c l i nic a l sample   P RI N C I P LE:   W e ca n d e te c t dif f e r e nt t y p e s of a nt i g e n a nd a nt i bo d y b a s e d on the a nt i g e n a nd a nt i bo d y in t e r a c t i on b y E L I S A. I n whi c h Dot E L I S A is a q u a l i tative t y pe o f test a n d d e te c t the a nt i g e n o r a nt i bo d y r a pid l y . . I n D o t - E L I S A, small volu me s of a nt i bodies a re i m mob i l i z e d on a p r otein bind i ng memb r a ne ( N i tr o ce l l ulose) a nd the other a nt i bo d y is l i nk e d to a n e n z y me Ho r s e r a dish p e r x oidase ( H R P ). The t e st a nt i g e n a t fi r st r e a c ts with the i m m obi l i z e d a nt i bo d y a nd lat e r with the e n z y me - l i nk e d a nt i bo d y . ...